Although platelet-rich plasma (PRP) is now widely used in regenerative medicine and\ndentistry, contradictory clinical outcomes have often been obtained. To minimize such di_erences\nand to obtain high quality evidence from clinical studies, the PRP preparation protocol needs to be\nstandardized. In addition, emphasis must be placed on quality control. Following our previous\nspectrophotometric method of platelet counting, in this study, another simple and convenient\nspectrophotometric method to determine platelet aggregation activity has been developed. Citrated\nblood samples were collected from healthy donors and used. After centrifugation twice, platelets\nwere suspended in phosphate buffered saline (PBS) and adenosine diphosphate (ADP)-induced\naggregation was determined using a spectrophotometer at 615 nm. For validation, platelets pretreated\nwith aspirin, an antiplatelet agent, or hydrogen peroxide (H2O2), an oxidative stress-inducing agent,\nwere also analyzed. Optimal platelet concentration, assay buffer solution, and representative time\npoint for determination of aggregation were found to be 50-100*104/microL, PBS, and 3 min after\nstimulation, respectively. Suppressed or injured platelets showed a significantly lower aggregation\nresponse to ADP. Therefore, it suggests that this spectrophotometric method may be useful in quick\nchair-side evaluation of individual PRP quality.
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